This plugin presents an easy-to-use, computationally efficient, two- and three-dimensional, feature point-tracking tool for the automated detection and analysis of particle trajectories as recorded by video imaging in cell biology.
The tracking process requires no apriori mathematical modelling of the motion, it is self-initialising, it discriminates spurious detections, and it can handle temporary occlusion as well as particle appearance and disappearance from the image region.
The plugin is well suited for video imaging in cell biology relying on low-intensity fluorescence microscopy.
It allows the user to visualize and analyze the detected particles and found trajectories in various ways:
Preview and save detected particles for separate analysis
Global non progressive view on all trajectories
Focused progressive view on individually selected trajectory
Focused progressive view on trajectories in an area of interest
It also allows the user to find trajectories from uploaded particles position and information text files and then to plot particles parameters vs. time - along a trajectory
The plugin implements the feature point detection and tracking algorithm as described in:
I. F. Sbalzarini and P. Koumoutsakos.
Feature point tracking and trajectory analysis for video imaging in cell biology.
J. Struct. Biol., 151(2): 182-195, 2005.
Any publications that made use of this plugin should cite the above reference.
This helps to ensure the financial support of our project at ETH and will enable us to provide further updates and support.
Thanks for your help!
IN NO EVENT SHALL THE ETH BE LIABLE TO ANY PARTY FOR DIRECT, INDIRECT, SPECIAL, INCIDENTAL, OR CONSEQUENTIAL DAMAGES, INCLUDING LOST PROFITS, ARISING OUT OF THE USE OF THIS SOFTWARE AND ITS DOCUMENTATION, EVEN IF THE ETH HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. THE ETH SPECIFICALLY DISCLAIMS ANY WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE SOFTWARE PROVIDED HEREUNDER IS ON AN "AS IS" BASIS, AND THE ETH HAS NO OBLIGATIONS TO PROVIDE MAINTENANCE, SUPPORT, UPDATES, ENHANCEMENTS, OR MODIFICATIONS.